英语翻译本研究人以人结肠癌HT-29细胞为研究对象,探讨大蒜素诱导结肠癌HT-29细胞凋亡的作用,为研究结肠细胞癌的临床耐药性奠定基础.采用台盼蓝拒染法检测不同浓度大蒜素对人结肠癌HT-29

英语翻译本研究人以人结肠癌HT-29细胞为研究对象,探讨大蒜素诱导结肠癌HT-29细胞凋亡的作用,为研究结肠细胞癌的临床耐药性奠定基础.采用台盼蓝拒染法检测不同浓度大蒜素对人结肠癌HT-29
英语翻译
本研究人以人结肠癌HT-29细胞为研究对象,探讨大蒜素诱导结肠癌HT-29细胞凋亡的作用,为研究结肠细胞癌的临床耐药性奠定基础.采用台盼蓝拒染法检测不同浓度大蒜素对人结肠癌HT-29细胞增殖的抑制率；光镜,荧光显微镜观察HT-29细胞形态学变化,DNA琼脂糖凝胶电泳检测细胞DNA片段化情况；流式细胞术检测细胞线粒体膜电位的改变；流式细胞术分析细胞的凋亡率及细胞周期分布的变化.台盼蓝拒染法显示大蒜素作用细胞24h,48h,72h后均可抑制细胞增殖,且作用48小时后出现典型的凋亡形态学变化和生化改变；细胞线粒体膜电位有所下降且讲细胞阻滞于G1期,G2和S期有明显上升.流式细胞术测定结果显示 23浓度下大蒜素可明显诱导HT-29细胞凋亡.大蒜素对人结肠癌HT-29细胞增殖抑制作用可能是通过细胞周期阻滞和凋亡相关蛋白实现的.
近年来,随着肿瘤成为全国范围内第一的导致人口死亡的疾病种类.这为肿瘤药物需求提供一个庞大的市场,同时随着分子生物学相关技术的高速发展,也使抗肿瘤药物的研究与开发进入了一个新时代.

英语翻译本研究人以人结肠癌HT-29细胞为研究对象,探讨大蒜素诱导结肠癌HT-29细胞凋亡的作用,为研究结肠细胞癌的临床耐药性奠定基础.采用台盼蓝拒染法检测不同浓度大蒜素对人结肠癌HT-29
The researchers to colon cancer HT-29 cell as the research object, this paper discusses the garlic induction colon cancer HT-29 cell apoptosis function, for the research of the colon cancer cells clinical resistance lay the foundation. The sets out to test different concentrations RanFa blue garlic to colon cancer HT-29 cell proliferation inhibition rate; Light, fluorescence microscope HT-29 cell morphology change, DNA agarose gel electrophoresis cells of DNA fragments; Flow cytometry detection cells the mitochondrial membrane potential was the change; Flow cytometry was analyzed cell apoptosis rate and the distribution of the cell cycle changes. A wish to RanFa showed blue garlic role cells 24 h, 48 h, 72 h could be inhibiting cell proliferation, and the effect after 48 hours of morphological changes in typical apoptosis and biochemical changes; Cell the mitochondrial membrane potential fell and speak cell block in the G1 phase, G2 and S phase has increased dramatically. Flow cytometry determination results show that was 23 concentrations in garlic can obviously induction HT-29 cell apoptosis. Garlic to colon cancer HT-29 cell proliferation inhibition may be through the cell cycle arrest and apoptosis related proteins to realize.
In recent years, as the tumor become nationwide first to the death of the population disease species. This drug for cancer needs to provide a huge market, at the same time as molecular biology technology related to the high speed development, also make the antitumor drug research and development has entered a new era.